Key result
The N terminus, but not the C terminus, of myosin light chain kinase is necessary for high affinity binding to actomyosin-containing filaments in smooth and nonmuscle cells.
Population
Smooth and nonmuscle cells, detergent-washed smooth muscle contractile proteins, and recombinant truncated…
Comparison
Truncated myosin light chain kinases lacking N… vs Recombinant full-length myosin light chain…
Design
Preclinical
Authors
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N-terminal MLCK targeting warrants further mechanistic study in smooth muscle; leaves open translation to human cardiovascular therapies.
The N-terminal region, rather than the C-terminal region, of myosin light chain kinase is essential for its high-affinity binding to actomyosin filaments in smooth and nonmuscle cells.
Lin et al. (1997) studied this question. Truncated myosin light chain kinases (N and/or C termini) vs. Full-length myosin light chain kinase was evaluated on Binding to actomyosin-containing filaments. The N terminus, but not the C terminus, of myosin light chain kinase is necessary for high affinity binding to actomyosin-containing filaments in smooth and nonmuscle cells.
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