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June 23, 2012AJP Heart and Circulatory PhysiologyOpen Access

Internal defibrillation shocks caused electroporation near the electrode, with significantly greater propidium iodide uptake in the left ventricular epicardium of infarcted rabbit hearts (P<0.01).

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Why the study?

Does a 45-min recovery period after a 300-V defibrillation shock affect electroporation injury in normal and MI rabbit hearts?

Population

Langendorff-perfused rabbit hearts with and without chronic left ventricular myocardial infarction

Comparison

Single 300-V monophasic truncated exponential… vs Normal vs MI hearts; with vs without 45-min…

Design

Preclinical

Follow-up

45 minutes

Key result

Internal defibrillation shocks caused electroporation near the electrode, with significantly greater propidium iodide uptake in the left ventricular epicardium of infarcted rabbit hearts (P<0.01).

Authors

YWYves T. WangIEIgor R. EfimovYCYuanna Cheng

Discussion

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Overview

Electroporation injury from defibrillation may be amplified in infarcted myocardium; hypothesis-generating for recovery timelines and clinical translation.

Structured PICO

Does a 45-min recovery period after a 300-V defibrillation shock affect electroporation injury in normal and MI rabbit hearts?

P
Population
31 Langendorff-perfused rabbit hearts with and without chronic left ventricular myocardial infarction.
I
Intervention
Single 300-V monophasic truncated exponential (MTE) shock (twice determined DFT voltage) to terminate sustained ventricular fibrillation, with or without a 45-min postshock recovery period
C
Comparator
Normal vs MI hearts; with vs without 45-min recovery period
O
Outcome
Shock-induced electroporation assessed by propidium iodide (PI) uptakesurrogate

Main Result

p-value: p=<0.01

Defibrillation shocks cause electroporation concentrated near the shock electrode, with increased injury in the infarct border zone of MI hearts, and recovery of membrane integrity is a prolonged process.

Cite This Study

Wang et al. (2012) studied Myocardial infarction and ventricular fibrillation (n=31). Internal defibrillation shock vs. Normal hearts / without recovery was evaluated on Shock-induced electroporation assessed by propidium iodide (PI) uptake (p=<0.01). Internal defibrillation shocks caused electroporation near the electrode, with significantly greater propidium iodide uptake in the left ventricular epicardium of infarcted rabbit hearts (P<0.01).

synapsesocial.com/papers/6a6c210647a1bfb288f36ad4https://doi.org/10.1152/ajpheart.01121.2011
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