Key result
19F NMR spectroscopy using 4F-BAPTA successfully measured simultaneous multi-compartment intracellular free [Ca2+] in intact perfused rabbit hearts.
19F NMR spectroscopy with 4F-BAPTA enables simultaneous measurement of cytosolic and sarcoplasmic reticulum calcium levels in intact living heart tissue.
Enables multi-compartment Ca2+ measurement in intact hearts; leaves open in vivo validation and clinical translation.
Although Ca2+ transport regulation at subcellular organelles is of great interest, only limited methodology has been available for measuring organellar [Ca2+] levels. The present study employs the 19F NMR resonance frequency of 4F-BAPTA to measure free [Ca2+]. In 4F-BAPTA loaded perfused rabbit hearts, two 19F NMR resonances were clearly observed. The frequency of one was consistent with cytosolic [Ca2+] levels. Responses to agents that after sarcoplasmic reticulum function identified the other resonance as originating from that organelle. The experiments demonstrate the utility of NMR shift indicator methodology in obtaining simultaneous multi-compartment intracellular [Ca2+] measurements and in enabling organellar [Ca2+] measurements to be made from within intact living tissue.
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Yanagida et al. (1996) studied this question. 19F NMR spectroscopy with 4F-BAPTA was evaluated on Measurement of free [Ca2+] in multiple compartments. 19F NMR spectroscopy using 4F-BAPTA successfully measured simultaneous multi-compartment intracellular free [Ca2+] in intact perfused rabbit hearts.
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