May 1, 1990Circulation ResearchOpen Access

Quantification of [Ca2+]i in perfused hearts. Critical evaluation of the 5F-BAPTA and nuclear magnetic resonance method as applied to the study of ischemia and reperfusion.

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Structured PICO

Population

Perfused ferret hearts

Intervention

20 minutes of total global ischemia at 30 degrees C followed by reperfusion, with [Ca2+]i measured using 5F-BAPTA and nuclear magnetic resonance spectroscopy

Comparator

Control levels (pre-ischemia)

Outcome

Time-averaged intracellular calcium concentration ([Ca2+]i)surrogate

The 5F-BAPTA and NMR method effectively measures intracellular calcium dynamics, demonstrating that [Ca2+]i rises during ischemia and normalizes shortly after reperfusion in perfused hearts.

Limitations

Nonlinear response of 5F-BAPTA to [Ca2+] leads to underestimation of the true time-averaged [Ca2+]i

Discussion

No takes yet. Share an insight, caveat, or question.

Implication

Preclinical calcium dynamics in ischemia leave open whether targeting [Ca2+]i improves human reperfusion outcomes.

Cite This Study

Marbán et al. (1990) studied this question.

synapsesocial.com/papers/6a718b7a5d37378ac1deab69 https://doi.org/10.1161/01.res.66.5.1255

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Quantification of [Ca2+]i in perfused hearts. Critical evaluation of the 5F-BAPTA and nuclear magnetic resonance method as applied to the study of ischemia and reperfusion.

Structured PICO

Limitations

Discussion

Implication

Cite This Study

Quantification of [Ca2+]i in perfused hearts. Critical evaluation of the 5F-BAPTA and nuclear magnetic resonance method as applied to the study of ischemia and reperfusion.

Structured PICO

Limitations

Discussion

Implication

Cite This Study

Also Consider

Also Consider