5/6 nephrectomy induces different renal, cardiac and vascular consequences in 129/Sv and C57BL/6JRj mice

Figures

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  Figure 1. Evolution of body weight (A,B) and urine output (C,D) from week 0 (W0; 5/6 Nx) to W12 (sacrifice) in sham-operated and 5/6 Nx C57BL/6JRj (A,C) and 129/Sv (B,D) mice (n = 10 per group). *p < 0.05: sham vs. 5/6 Nx, **p < 0.01: sham vs. 5/6 Nx, ***p < 0.001: vs. 5/6 Nx. Plasma creatinine at week 12 (E) and aldosteronuria (F) in sham-operated and 5/6 Nx C57BL/6JRj and 129/SV mice (n = 8–9 per group). *p < 0.05: sham vs 5/6 Nx, ***p < 0.001: sham vs 5/6 Nx.

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  Figure 2. Scoring of kidney lesions 12 weeks after surgery in sham-operated and 5/6 Nx C57BL/6JRj and 129/Sv mice (n = 6–10 per group). (A) Glomerular sclerosis (GS) lesions, (B) Tubular damage (TD) lesions, (C) Interstitial fibrosis (IF) lesions, (D) Interstitial inflammation (II) lesions, (E) Vascular thickening (VT) lesions, (F) Vascular fibrosis (VF) lesions. ***p < 0.001: sham vs 5/6 Nx, $p < 0.05: 5/6 Nx (129/Sv) vs. 5/6 Nx (C57BL/6JRj), $$$p < 0.001: 5/6 Nx (129/Sv) vs. 5/6 Nx (C57BL/6JRj).

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  Figure 3. Evolution of systolic blood pressure from week 0 (W0; 5/6 Nx) to W12 (sacrifice) in sham-operated and 5/6 Nx C57BL/6JRj (A) and 129/SV (B) mice (n = 10 per group). *p < 0.05: sham vs. 5/6 Nx, ***p < 0.001: vs. 5/6 Nx. Heart weight (w) to tibia length ratio (C) and morphometric quantification of left ventricular cardiac fibrosis determined 12 weeks after surgery (D) in sham-operated and 5/6 Nx C57BL/6JRj and 129/SV mice (B) (n = 7–9 per group). **p < 0.01: sham vs. 5/6 Nx, $$p < 0.01: 5/6 Nx (129/Sv) vs. 5/6 Nx (C57BL/6JRj), ***p < 0.001: sham vs. 5/6 Nx, $$$p < 0.001: 5/6 Nx (129/Sv) vs. 5/6 Nx (C57BL/6JRj).

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  Figure 4. Relaxing responses of pre-contracted mesenteric arteries in response to acetylcholine (A,B) and sodium nitroprusside (C,D) in sham-operated and 5/6 Nx C57BL/6JRj (A,C) and 129/Sv (B,D) mice (n = 7–8 per group). Dilatory responses of pre-constricted mesenteric arteries in response to stepwise increase in intraluminal flow in sham-operated and 5/6 Nx C57BL/6JRj (E) and 129/Sv (F) mice (n = 7–8 per group). *p < 0.05: sham vs. 5/6 Nx, **p < 0.01: sham vs. 5/6 Nx, ***p < 0.001: sham vs. 5/6 Nx.