Ventricular cells exhibited an average of 2.32 overlapping open inward rectifier K channels per patch, compared to 0.03-0.06 in nodal cells, explaining the lower resting K conductance in pacemaker cells.
Absolute Event Rate: 2.32% vs 0.06%
Currents through the inward rectifier K channel (iK X rec) and the ACh-operated K channel (iK X ACh) were recorded in isolated heart cells of rabbit using the patch clamp technique with electrodes having 0.5-1 micron tip inner diameter. The maximum number of overlaps of open iK X rec channels per patch was measured over 347 experiments. An average of 2.3 was found in ventricular cells and 0.03-0.06 in sinoatrial (S-A) and atrioventricular (A-V) node cells. The estimated total number of the iK X rec channels for each ventricular cell was great enough to supply the resting K conductance of the cell. The iK X ACh channel was present in S-A and A-V node cells, but was never observed in the ventricular cells. The resting conductance of the nodal cells measured with whole cell clamp recordings was about 16 times smaller than that of the ventricular cells, and was hardly decreased at all by the removal of K+ from the bath solution. Thus, the lower membrane potential of the nodal cells compared with that of the ventricular cells was attributed to the smaller K conductance of the resting membrane, which is due to the very low density of the iK X rec channel. On the other hand, the iK X ACh channel, when activated by neural regulation, may play a major role in generating the resting K conductance of the nodal cells.
Noma et al. (Sun,) conducted a other in Normal cardiac electrophysiology (n=347). Ventricular cells vs. Sinoatrial and atrioventricular node cells was evaluated on Average number of overlaps of open inward rectifier K channels (iK.rec) per patch. Ventricular cells exhibited an average of 2.32 overlapping open inward rectifier K channels per patch, compared to 0.03-0.06 in nodal cells, explaining the lower resting K conductance in pacemaker cells.