Potential Antimicrobial Efficacy and Phytochemical Screening Of Oleoresin Extract FromChir-Pine Against Bacterial Pathogens

In present study, antimicrobial, antioxidant and phytochemical activities of Oleoresin from Chir-pine was carried out with the help of Agar well diffusion method. The antimicrobial activities of different concentrations of Oleoresin against Staphylococcus aureus, Escherichia coli, K. pneumoniae, Bacillus paramycoides, Enterobacter aerogens, Corynebacterium striatum, Pseudomonas aeruginosa at two different concentrations Viz. 150 mg/ml, and 200 mg/ml were tested. On the other hand, antioxidant activity of Oleoresin was also tested. High antimicrobial activity was recorded at 200 mg/ml concentration of extract with the inhibitory zone of 24± 0.34 cm against Pseudomonas aeruginosa whereas minimum activity was recorded against Bacillus paramycoides at 200 mg/ml with the inhibitory zone of 11± 0.30 cm whereas at 150 mg/ml concentration of extract minimum zone of inhibition was recorded against Pseudomonas aeruginosa, (11± 0.35 Cm), Bacillus paramycoides (11± 0.30 Cm), Escherichia coli (11 ± 0.35 Cm). Phytochemical screening revealed the presence of Carbohydrates, Phenolic compounds, Steroid along with terpenoid and absence of Saponin glycosides, Flavoniodes, Insulin and Amino acid. Further significant amount of Rosin %, α - Pinene, β - Pinene , Carene and Turpentine Oil % was present in Oleoresin obtained from Chir-pine which provides significant antimicrobial activity against the bacterial pathogens.