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Inhibitors of translation (cycloheximide, puromycin) in cultured H-35 cells suggest that bound ribosomes stabilize messenger RNA in vivo and its degradation rate is determined by translation rate.
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In mammalian cells, bound ribosomes stabilize mRNA in vivo, and the degradation rate of mRNA is determined by the rate of translation.
Analyses of the response of hydrocortisone-induced tyrosine transaminase in cultured H-35 cells to inhibitors of translation (cycloheximide, puromycin) suggest: (1) that bound ribosomes stabilize messenger RNA in vivo ; (2) that messenger is degraded at a rate determined by the rate of translation. Since specific messenger RNAs of mammalian cells are degraded at quite different rates, there may be extensive heterogeneity either in the rate at which ribosomes traverse different messengers or in the number of ribosomes which translate specific messenger RNAs.
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Kenney et al. (1972) studied Cultured H-35 cells. Inhibitors of translation (cycloheximide, puromycin) was evaluated on Degradation rate of messenger RNA. Inhibitors of translation (cycloheximide, puromycin) in cultured H-35 cells suggest that bound ribosomes stabilize messenger RNA in vivo and its degradation rate is determined by translation rate.
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