Key result
Cdc42 binding to the GBD-C protein increased the amide hydrogen exchange rates of the most protected amides by 50-500-fold, facilitating the physical separation of GBD from VCA.
Effect estimate: 50-500-fold increase
Cdc42 binding destabilizes the autoinhibited conformation of WASP, facilitating the physical separation of GBD from VCA, though destabilization is incomplete, suggesting multiple signals are needed for maximal activation.
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Hypothesis-generating for WASP regulation; leaves open cellular and in vivo validation.
Buck et al. (2001) studied this question. Cdc42 binding vs. Free state (unbound) was evaluated on Amide hydrogen exchange rates (50-500-fold increase). Cdc42 binding to the GBD-C protein increased the amide hydrogen exchange rates of the most protected amides by 50-500-fold, facilitating the physical separation of GBD from VCA.
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