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April 5, 2022Frontiers in Cardiovascular MedicineOpen Access

Assessment of Myocardial Microstructure in a Murine Model of Obesity-Related Cardiac Dysfunction by Diffusion Tensor Magnetic Resonance Imaging at 7T

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Key result

Fifteen weeks of high-fat diet in mice induced obesity-related diastolic dysfunction and reduced global longitudinal strain (-15.1% vs -20.0%), accompanied by lower mean diffusivity in the left-ventricular base.

Why the study?

Obesity causes multiple deleterious effects on the heart that may lead to cardiac failure, prompting characterization of myocardial microstructure and function in an experimental model of obesity-related cardiac dysfunction.

Does a high-fat diet induce alterations in myocardial microstructure and function in a murine model?

Comparison

High-fat diet (60 kcal% fat, n = 12) vs standard control diet (9 kcal% fat, n = 10)

Design

Animal experimental study

Follow-up

15 weeks

Authors

DLDavid LohrATArne ThieleMSMax Stahnke

Discussion

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Member takes

Overview

Characterizes myocardial changes in obese mice; leaves open translation to human obesity-related cardiomyopathy.

Structured PICO

Does a high-fat diet induce alterations in myocardial microstructure and function in a murine model?

P
Population
21 male C57BL/6N mice, 8-9 weeks old, were fed either a high-fat diet or standard control diet for 15 weeks to evaluate obesity-related cardiac dysfunction.
I
Intervention
High-fat diet (HFD; 60 kcal% fat) for 15 weeks
C
Comparator
Standard control diet (9 kcal% fat) for 15 weeks
O
Outcome
Myocardial microstructure and function assessed by ultra-high frequency echocardiography and diffusion tensor magnetic resonance imaging (DT-MRI) at 7Tsurrogate

Main Result

Absolute Event Rate: -15.1% vs -20%

p-value: p=0.01

Diet-induced obesity in mice leads to diastolic dysfunction and regional alterations in myocardial diffusion properties before the onset of fibrosis or three-dimensional myofiber remodeling.

Limitations

  • Relatively small sample size studied by DT-MRI due to long acquisition times and complex post-processing
  • Epicardial adipose tissue was removed during sample preparation, preventing assessment of its impact on cardiac diffusion properties
  • Small size of the murine heart limits layer-specific strain analysis to the endocardial and epicardial borders
  • Lack of a validated structural correlate for the observed decrease in diffusivity
  • Fixation with formalin affects diffusion properties
  • Cardiac phenotype may be confounded by mild glucose intolerance

Cite This Study

Lohr et al. (2022) studied Obesity-related cardiac dysfunction (n=21). High-fat diet vs. Standard control diet (9 kcal% fat) was evaluated on Global longitudinal strain (p=0.01). Fifteen weeks of high-fat diet in mice induced obesity-related diastolic dysfunction and reduced global longitudinal strain (-15.1% vs -20.0%), accompanied by lower mean diffusivity in the left-ventricular base.

synapsesocial.com/papers/6a6cb7ba8031ec7bb1db47cbhttps://doi.org/10.3389/fcvm.2022.839714
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Characterization of Myocardial Microstructure and Function in an Experimental Model of Isolated Subendocardial Damage2019 · 35 citations
  2. 2Impact of Epicardial Adipose Tissue, Left Ventricular Myocardial Fat Content, and Interstitial Fibrosis on Myocardial Contractile Function2018 · 141 citations
  3. 3Evidence Supporting the Existence of a Distinct Obese Phenotype of Heart Failure With Preserved Ejection Fraction2017 · 1,162 citations
  4. 4Prominent longitudinal strain reduction of left ventricular basal segments in treatment-naïve Anderson-Fabry disease patients2018 · 51 citations
  5. 5Effects of Bariatric Surgery on Cardiac Structure and Function: A Systematic Review and Meta-Analysis2013 · 118 citations