Key result
Monoclonal antibody 3G12 against NPR-B inhibited CNP-stimulated cGMP synthesis and increased the IC50 for CNP-mediated relaxation of rat aortic rings from 10 nM to 140 nM.
Population
Cells isolated from media or adventitia layers of rat thoracic aorta, and phenylephrine-contracted rat…
Comparison
Monoclonal antibody 3G12 against natriuretic… vs Control (absence of 3G12)
Design
Preclinical
Authors
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Antibody tools for NPR-B enable mechanistic studies; leaves open therapeutic modulation in cardiovascular disease.
This study demonstrates for the first time that C-type natriuretic peptide (CNP) relaxes vascular smooth muscle specifically through binding to the natriuretic peptide receptor-B (NPR-B).
Drewett et al. (1995) studied this question. Monoclonal antibody 3G12 against NPR-B was evaluated on Relaxation of phenylephrine-contracted rat aortic rings and cGMP synthesis. Monoclonal antibody 3G12 against NPR-B inhibited CNP-stimulated cGMP synthesis and increased the IC50 for CNP-mediated relaxation of rat aortic rings from 10 nM to 140 nM.
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