⌘+K

Pulse Explore Clubs Researchers Journals

Home Clubs Explore

March 1, 1999Arteriosclerosis Thrombosis and Vascular Biology

Analysis of Macrophage Scavenger Receptor (SR-A) Expression in Human Aortic Atherosclerotic Lesions

View Full Paper

Ask AI

Bookmark

Share

Authors

PGPeter J. GoughAge UK DGDavid R. GreavesUniversity of Oxford HSHiroshi SuzukiNihon University

Discussion

Loading...

Member takes

Implication

Laboratory study demonstrates selective SR-A expression in human atherosclerotic foam cells and tissue macrophages, suggesting a primary role in lipid uptake during plaque formation.

Key Points

To generate specific antibodies recognizing human scavenger receptor A (SR-A) and characterize its expression and functional role in human cells and atherosclerotic tissue.
Generated high-titer, cross-reactive polyclonal antisera against human SR-A by immunizing SR-A knockout (SR-A -/-) mice.
Assessed SR-A expression across monocyte-to-macrophage differentiation, healthy tissues, and human aortic atherosclerotic lesions using multiple immunological techniques.
Measured the proportion of acetylated low-density lipoprotein uptake mediated by SR-A in human monocyte-derived macrophages.
SR-A-deficient mice produced high-titer antisera recognizing both type I and II SR-A across human, mouse, and rabbit species.
SR-A expression was induced during monocyte-to-macrophage differentiation and accounted for 80% of acetylated low-density lipoprotein uptake in human monocyte-derived macrophages.
Human SR-A localized strongly to macrophage-derived foam cells in aortic atherosclerotic lesions and tissue macrophages in liver and lung, with minimal detection in smooth muscle cells or endothelium.

Cite This Study

Gough et al. (1999) studied this question.

synapsesocial.com/papers/6a6cf0ffa7fbea1e44078e64 https://doi.org/10.1161/01.atv.19.3.461

View Full Paper

Ask AI

Bookmark

Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

1Factors regulating the activities of the low density lipoprotein receptor and the scavenger receptor on human monocyte-macrophages.1981 · 231 citations
2Expression of LDL Receptor, VLDL Receptor, LDL Receptor–Related Protein, and Scavenger Receptor in Rabbit Atherosclerotic Lesions1998 · 158 citations
3Expression of the Macrophage Scavenger Receptor in Atheroma1995 · 106 citations
4Rabbit aortic smooth muscle cells express inducible macrophage scavenger receptor messenger RNA that is absent from endothelial cells.1992 · 124 citations
5Structure, organization, and chromosomal mapping of the human macrophage scavenger receptor gene.1993 · 134 citations