Key result
Amlodipine (10-1,000 nM) inhibited thrombin- and thapsigargin-induced Ca(2+) mobilization from internal stores in vascular smooth muscle cells, an effect not seen with other Ca(2+) channel blockers.
Why the study?
Does amlodipine inhibit calcium mobilization in thrombin- and thapsigargin-stimulated vascular smooth muscle cells?
Population
Fura 2-loaded cultured vascular smooth muscle cells (VSMCs) stimulated with thrombin and thapsigargin
Comparison
Amlodipine (10-1,000 nM) vs Isradipine, diltiazem, or verapamil (100 nM)
Design
Preclinical
Authors
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May inform VSMC Ca2+ mechanisms in vitro; leaves open clinical relevance and should not change practice.
Does amlodipine inhibit calcium mobilization in thrombin- and thapsigargin-stimulated vascular smooth muscle cells?
Amlodipine specifically inhibits calcium mobilization from internal stores in vascular smooth muscle cells, independent of L-type calcium channel blockade, potentially contributing to its inhibitory effect on cell growth.
Stepien et al. (2000) studied this question. Amlodipine vs. 100 nM isradipine, diltiazem, or verapamil was evaluated on Variations of intracellular free Ca(2+) concentration in fura 2-loaded cultured VSMCs. Amlodipine (10-1,000 nM) inhibited thrombin- and thapsigargin-induced Ca(2+) mobilization from internal stores in vascular smooth muscle cells, an effect not seen with other Ca(2+) channel blockers.
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