Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
May 15, 2014Platelets

Compartmentalisation of cAMP-dependent signalling in blood platelets: The role of lipid rafts and actin polymerisation

View Full Paper
Ask AI
Bookmark
Share

Key result

Disruption of lipid rafts with MβCD or the actin cytoskeleton with cytochalasin D increased platelet sensitivity to PGI2 and forskolin, resulting in greater inhibition of platelet aggregation.

Population

Blood platelets

Comparison

Disruption of lipid rafts with… vs Untreated platelets

Design

Preclinical

Authors

ZRZaher RaslanRoche (United Kingdom)KNKhalid M. NaseemUniversity of Leeds

Discussion

Loading...

Member takes

Overview

Should not yet influence antiplatelet therapy; extends basic understanding of cAMP compartmentalization in platelets.

Structured PICO

P
Population
Blood platelets
I
Intervention
Disruption of lipid rafts with methyl-beta-cyclodextrin (MβCD) or actin cytoskeleton with cytochalasin D
C
Comparator
Untreated platelets
O
Outcome
Platelet aggregation, cAMP formation, and PKA-mediated signalling eventssurrogate

Lipid rafts and the actin cytoskeleton play a key role in restraining basal cAMP signalling and regulating the inhibitory effects of prostacyclin in blood platelets.

Cite This Study

Raslan et al. (2014) studied this question. Disruption of lipid rafts with methyl-beta-cyclodextrin (MβCD) or actin cytoskeleton with cytochalasin D was evaluated on Platelet sensitivity to PGI2 and forskolin (cAMP formation, PKA-mediated signalling, platelet aggregation). Disruption of lipid rafts with MβCD or the actin cytoskeleton with cytochalasin D increased platelet sensitivity to PGI2 and forskolin, resulting in greater inhibition of platelet aggregation.

synapsesocial.com/papers/6a6d6a35f44fa9f079dbaaadhttps://doi.org/10.3109/09537104.2014.916792
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Initial accumulation of platelets during arterial thrombus formation in vivo is inhibited by elevation of basal cAMP levels2003 · 83 citations
  2. 2Microtubules and Actin Microfilaments Regulate Lipid Raft/Caveolae Localization of Adenylyl Cyclase Signaling Components2006 · 261 citations
  3. 3The first comprehensive and quantitative analysis of human platelet protein composition allows the comparative analysis of structural and functional pathways2012 · 795 citations
  4. 4Genome-wide RNA-seq analysis of human and mouse platelet transcriptomes2011 · 576 citations
  5. 5Combined Spatial and Enzymatic Regulation of Csk by cAMP and Protein Kinase A Inhibits T Cell Receptor Signaling2003 · 76 citations