6-hydroxydopamine and ovariectomy has no effect on heart rate variability parameters of females

Figures

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  Figure 1. Number of TH+ neurons in the SNpc 40 days after striatal injection of 6-OHDA (A) Position of the CPu and SNpc in relation to the Bregma. (B) Coronal view of the SNpc and (C) photomicrographs of the SNpc neurons TH+ stained. (D) The number of TH+ neurons in the SNpc represented as Median±interquartile range. Data are analyzed using the Kruskal-Wallis test and Dunn-Bonferroni post-hoc test. *p<0.001 compared to Sham **p<0.001 compared to Sham+OVX. Sham, n=9; Sham+OVX, n=5; 6-OHDA, n=6; 6-OHDA+OVX, n=5. (SNpc, substantia nigra pars compacta; SNpr, substantia nigra pars reticulata; VTA, ventral tegmental area; TH, tyrosine hydroxylase; 6-OHDA, 6-hydroxydopamine; OVX, ovariectomized).

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  Figure 2. Heart rate variability parameters (time domain analysis) (A) Electrocardiographic signal for each group. (B) Heart rate (HR), (C) the R-R interval (RRi), (D) the Q and T interval (QTc), (E) the standard deviation of all normal-to-normal intervals (SDNN), and (F) the square root of the mean of the squared differences between adjacent normal RRi (RMSSD). Data are expressed as mean±standard error of the mean. Data are analyzed using GLM multivariate with the Sidak post-hoc test. Sham, n=4; Sham+OVX, n=5; 6-OHDA, n=5; 6-OHDA+OVX, n=5 (6-OHDA, 6-hydroxydopamine; OVX, ovariectomized).

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  Figure 3. Frequency domain analysis of heart rate variability (A) LF. (B) HF. (C) LF/HF. Data are expressed as mean ± standard error of the mean. Data are analyzed using GLM multivariate with the Sidak post-hoc test. (LF, the low-frequency component in normalized units; HF, the high-frequency component in normalized units; LF/HF, the ratio between LF and HF; 6-OHDA, 6-hydroxydopamine; OVX, ovariectomized).

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  Figure 4. RRi distribution analysis (A) Representation of the skewness measure in a distribution. (B) Representation of kurtosis in a distribution. (C) Skewness. (D) Kurtosis. Data are expressed as mean ± standard error of the mean. Data are analyzed using GLM multivariate with the Sidak post-hoc test (6-OHDA, 6-hydroxydopamine; OVX, ovariectomized).