Key result
Expression of the rat heart I alpha-subunit in Xenopus oocytes produced Na+ currents with kinetic properties and tetrodotoxin resistance characteristic of native cardiac Na+ channels.
Population
Xenopus oocytes injected with cRNA transcribed from the rat heart I cDNA clone
Design
Preclinical
Authors
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Enables molecular studies of cardiac Na+ channels; leaves open in vivo validation and therapeutic relevance.
The rat heart I alpha-subunit primary amino acid sequence is sufficient to express tetrodotoxin-resistant Na+ channels characteristic of cardiac muscle.
Cribbs et al. (1990) studied this question. Injection of cRNA transcribed from the rat heart I cDNA clone was evaluated on Kinetic properties and resistance to tetrodotoxin and saxitoxin. Expression of the rat heart I alpha-subunit in Xenopus oocytes produced Na+ currents with kinetic properties and tetrodotoxin resistance characteristic of native cardiac Na+ channels.
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