What question did this study set out to answer?

Investigate how Saccharomyces cerevisiae coordinates the reciprocal expression of aerobic (CWP) and anaerobic (DAN/TIR) cell wall mannoprotein genes in response to oxygen availability and temperature stress.

May 1, 2001Journal of BacteriologyOpen Access

Reciprocal Regulation of Anaerobic and Aerobic Cell Wall Mannoprotein Gene Expression in Saccharomyces cerevisiae

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Authors

NANatalia E. AbramovaAlbany Medical Center Hospital OSOdeniel SertilAlbany Medical Center Hospital SMSameer MehtaPearson (United States)

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Implication

Gene expression analysis reveals reciprocal regulation of cell wall mannoproteins in Saccharomyces cerevisiae, indicating programmed cell wall remodeling during oxygen deprivation.

Key Points

Investigate how Saccharomyces cerevisiae coordinates the reciprocal expression of aerobic (CWP) and anaerobic (DAN/TIR) cell wall mannoprotein genes in response to oxygen availability and temperature stress.
Monitored temporal gene expression of DAN, TIR, TIP1, and CWP genes following an anaerobic shift and cold shock.
Assessed the regulatory roles of the heme-responsive factors Mox4 and Mot3 on CWP2 and DAN/TIR expression.
Evaluated anaerobic growth phenotypes across targeted deletion mutants lacking TIR1, TIR3, or TIR4.
Oxygen depletion triggers induction of nine DAN/TIR mannoprotein genes within 1 to 3 hours alongside concurrent down-regulation of aerobic counterparts CWP1 and CWP2.
The transcription factors Mot3 and Mox4 mediate reciprocal regulation by acting in opposite directions on CWP2 compared to DAN/TIR genes.
Individual disruption of TIR1, TIR3, or TIR4 completely abolishes anaerobic growth, demonstrating their essential role in hypoxic adaptation.

Cite This Study

Abramova et al. (2001) studied this question.

synapsesocial.com/papers/6a6e0fe4f44fa9f079dbf657 https://doi.org/10.1128/jb.183.9.2881-2887.2001

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Implication

Gene expression analysis reveals reciprocal regulation of cell wall mannoproteins in Saccharomyces cerevisiae, indicating programmed cell wall remodeling during oxygen deprivation.

Reciprocal Regulation of Anaerobic and Aerobic Cell Wall Mannoprotein Gene Expression in Saccharomyces cerevisiae

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Key Points

Cite This Study

Reciprocal Regulation of Anaerobic and Aerobic Cell Wall Mannoprotein Gene Expression in Saccharomyces cerevisiae

Discussion

Member takes

Implication

Key Points

Cite This Study

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