What question did this study set out to answer?

Identify and characterize the heme-responsive regulatory factors and pathways that control DAN/TIR mannoprotein gene expression during anaerobic cell wall remodeling in yeast.

March 1, 2001GeneticsOpen Access

Regulatory Mechanisms Controlling Expression of the DAN/TIR Mannoprotein Genes During Anaerobic Remodeling of the Cell Wall in Saccharomyces cerevisiae

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Authors

NANatalia E. AbramovaAlbany Medical Center Hospital BCBrian D. CohenUnion College OSOdeniel SertilAlbany Medical Center Hospital

Discussion

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Implication

Molecular genetic study reveals multi-factor heme-responsive control of DAN/TIR mannoprotein expression in Saccharomyces cerevisiae, highlighting pathways coordinating anaerobic cell wall remodeling.

Key Points

Identify and characterize the heme-responsive regulatory factors and pathways that control DAN/TIR mannoprotein gene expression during anaerobic cell wall remodeling in yeast.
Screened recessive Saccharomyces cerevisiae mutants displaying altered expression of a dan1::ura3 reporter fusion under aerobic conditions.
Conducted epistasis and mutation analyses involving the transcription factors Mox1, Mox2, and Mox4/Upc2.
Assessed regulatory contributions of heme-responsive repressors Rox1 and Rox7 and global corepressors Tup1 and Ssn6.
Identified Mox4 (identical to the zinc cluster factor Upc2) as an essential transcriptional activator for all DAN/TIR genes acting through AR1 consensus sequences.
Observed that noninducible mox4-null mutations were epistatic to constitutive mox1 and mox2 mutations, showing Mox1 and Mox2 modulate Mox4 activation in a heme-dependent manner.
Found that full aerobic repression of DAN/TIR genes requires cooperative action among Rox7, Rox1, and the global corepressors Tup1 and Ssn6.

Cite This Study

Abramova et al. (2001) studied this question.

synapsesocial.com/papers/6a6e0fe4f44fa9f079dbf658 https://doi.org/10.1093/genetics/157.3.1169

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Implication

Molecular genetic study reveals multi-factor heme-responsive control of DAN/TIR mannoprotein expression in Saccharomyces cerevisiae, highlighting pathways coordinating anaerobic cell wall remodeling.