Key result
The 3B-cCLIA demonstrated high diagnostic sensitivity and specificity for detecting antibodies against FMDV non-structural protein 3B across swine, cattle, and sheep.
Why the study?
Anti-non-structural protein antibody detection is important for confirming suspected foot-and-mouth disease cases, evaluating infection prevalence, certifying animals for trade, and disease control.
Answers are grounded in this paper and cite their sources.
The newly developed 3B-cCLIA provides a rapid and accurate method for detecting FMDV NSP 3B antibodies across multiple livestock species, though repeated vaccination increases false-positive rates.
BACKGROUND: Foot-and-mouth disease (FMD) is a devastating animal disease. Anti-non-structural protein (NSP) antibody detection is very important for confirming suspected cases, evaluating the prevalence of infection, certifying animals for trade and controlling the disease. METHODS: In this study, a competitive chemiluminescence immunoassay (3B-cCLIA) was developed for the rapid detection of antibodies against NSPs in different species of livestock animals using the monoclonal antibody (mAb) 9E2 as a competitive antibody that recognizes NSP 3B. RESULTS: The cut-off value (50%), diagnostic sensitivity (Dsn) (97.20%, 95.71%, and 96.15%) and diagnostic specificity (Dsp) (99.51%, 99.43%, and 98.36) of the assay were estimated by testing a panel of known-background sera from swine, cattle and sheep, respectively. The accuracy rate of the 3B-cCLIA was further validated and subsequently compared with that of two commercial diagnostic kits. The early diagnostic results showed that antibodies recognizing NSPs developed later (approximately 1-2 days) than antibodies recognizing structural proteins. Furthermore, anti-NSP antibody presence in animals vaccinated multiple times (false positives), especially cattle and sheep, was confirmed, and the false-positive rate increased with the number of vaccinations. CONCLUSIONS: These results indicate that the 3B-cCLIA is suitable for the rapid detection of antibodies against FMDV NSP 3B in a wide range of species.
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Liu et al. (2021) studied Foot-and-mouth disease (n=875). 3B-cCLIA vs. 3ABC-bELISA and PrioCHECK FMDV NSP ELISA was evaluated on Diagnostic sensitivity and specificity. The 3B-cCLIA demonstrated high diagnostic sensitivity and specificity for detecting antibodies against FMDV non-structural protein 3B across swine, cattle, and sheep.
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