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A Western blotting assay based on baculovirus-expressed nonstructural proteins 2C and 3D successfully differentiated FMDV-infected pigs from vaccinated pigs by detecting antibodies only in the infected group.
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A western blotting assay using baculovirus-expressed nonstructural proteins 2C and 3D can successfully differentiate FMDV-infected pigs from vaccinated pigs.
Baculovirus-expressed foot-and-mouth disease virus (FMDV) nonstructural proteins 2C and 3D were used as the antigens in a western blotting assay. This assay allowed for differentiation of FMDV-infected pigs from vaccinated pigs. Serial studies were performed using sera collected from experimentally infected and vaccinated pigs. Positive reactions were found in the sera derived from the experimentally infected pigs. There was, however, no positive reaction in the vaccinated pigs. These results suggest that this western blotting assay is a useful method for differentiation of infected pigs from vaccinated pigs.
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Fukai et al. (2008) studied Foot-and-mouth disease (n=7). Western blotting assay using baculovirus-expressed NSPs 2C and 3D vs. Vaccinated pigs was evaluated on Detection of antibodies to FMDV nonstructural proteins 2C and 3D. A Western blotting assay based on baculovirus-expressed nonstructural proteins 2C and 3D successfully differentiated FMDV-infected pigs from vaccinated pigs by detecting antibodies only in the infected group.
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