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A newly developed chromatographic strip assay detected foot-and-mouth disease virus anti-3ABC antibodies in pigs with 96.8% sensitivity and 98.8% to 100% specificity, comparable to commercial ELISA kits.
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A newly developed chromatographic strip assay provides rapid, sensitive, and specific on-farm detection of foot-and-mouth disease virus antibodies in pigs, comparable to commercial ELISA kits.
A chromatographic strip assay was developed for rapid detection of serum antibodies to non-structural protein of foot-and-mouth disease virus. The assay was based on Escherichia coli-expressed 3ABC non-structural protein and an immunochromatographic technique, which shortened the detection time to about one hour. The sensitivity of the assay was determined to be 96.8% for infected pigs; its specificity was 100% for naïve pigs and 98.8% for vaccinated pigs. In the experimentally infected pigs, anti-3ABC antibodies were detectable from eight days post-infection until the end of the study, 34 days post-infection. The performance of this assay was comparable to that of two commercial ELISA kits, Ceditest FMDV-NS and UBI FMDV NS EIA, and was better than that of CHEKIT FMD-3ABC po. Given its advantages of instant testing and quantitative measurement, this assay has potential as a useful tool for rapid on-farm diagnosis of foot-and-mouth disease.
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Chen et al. (2009) studied Foot-and-mouth disease (FMD) (n=483). Chromatographic strip assay vs. Commercial ELISA kits was evaluated on Sensitivity and specificity for detecting anti-3ABC antibodies. A newly developed chromatographic strip assay detected foot-and-mouth disease virus anti-3ABC antibodies in pigs with 96.8% sensitivity and 98.8% to 100% specificity, comparable to commercial ELISA kits.
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