Key result
The Danish C-ELISA demonstrated 71% sensitivity and 90% specificity, while the CHEKIT ELISA was 23% sensitive and 98% specific for detecting foot-and-mouth disease antibodies compared to cVNT.
Why the study?
What is the diagnostic sensitivity and specificity of the CHEKIT ELISA and Danish C-ELISA for detecting FMDV antibodies in an unvaccinated cattle population compared to the virus neutralization test?
Cross-Sectional (n=1,377)
Yes
What is the diagnostic sensitivity and specificity of the CHEKIT ELISA and Danish C-ELISA for detecting FMDV antibodies in an unvaccinated cattle population compared to the virus neutralization test?
Answers are grounded in this paper and cite their sources.
The Danish C-ELISA demonstrated superior sensitivity compared to the commercially available CHEKIT ELISA for detecting FMDV antibodies in an endemic setting, though both tests had limitations in sensitivity, particularly in juvenile cattle.
The development of a serological test for foot-and-mouth disease virus (FMDV) which is quick and easy to use, which can identify all seven serotypes, and which can differentiate vaccinated from convalescing or potential virus carriers would be a major advance in the epidemiological toolkit for FMDV. The nonstructural polyprotein 3ABC has recently been proposed as such an antigen, and a number of diagnostic tests are being developed. This paper evaluates the performance of two FMDV tests for antibodies to nonstructural proteins in an unvaccinated cattle population from a region of Cameroon with endemic multiple-serotype FMD. The CHEKIT-FMD-3ABC bo-ov (CHEKIT) enzyme-linked immunosorbent assay (ELISA) (Bommeli Diagnostics/Intervet) is a commercially available test that was compared with a competitive 3ABC ELISA (C-ELISA) developed in Denmark. The tests were compared with the virus neutralization test as the "gold standard." Diagnostic sensitivity and specificity were examined over a range of test cutoffs by using receiver operating characteristic curves, which allowed comparison of the overall performance of each test. The results indicated that the CHEKIT ELISA kit was 23% sensitive and 98% specific and the Danish C-ELISA was 71% sensitive and 90% specific at the recommended cutoff. These results have important implications if the tests are to be used to screen herds or individual cattle in surveillance programs, at border crossings for import-export clearance, or following emergency vaccination in an outbreak situation.
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Bronsvoort et al. (2004) conducted a cross-sectional in Foot-and-Mouth Disease (n=1,377). 3ABC Enzyme-Linked Immunosorbent Assays (CHEKIT ELISA and Danish C-ELISA) vs. Combined virus neutralization test (cVNT) was evaluated on Diagnostic sensitivity and specificity at recommended cutoffs. The Danish C-ELISA demonstrated 71% sensitivity and 90% specificity, while the CHEKIT ELISA was 23% sensitive and 98% specific for detecting foot-and-mouth disease antibodies compared to cVNT.
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