January 29, 2002Circulation
Electrophysiological Modulation of Cardiomyocytic Tissue by Transfected Fibroblasts Expressing Potassium Channels
Key result
Transfected fibroblasts expressing Kv1.3 caused a significant, reversible reduction in extracellular signal amplitude and multiple local conduction blocks in cardiomyocytic cultures.
Why the study?
Does the addition of transfected fibroblasts expressing Kv1.3 modify the electrophysiological properties of cardiomyocytic cultures?
Population
Primary cultures of neonatal rat ventricular myocytes
Comparison
Transfected fibroblasts expressing the… vs Nontransfected fibroblasts added to the…
Design
Preclinical
Authors
Discussion
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Implication
Hypothesis-generating for Kv1.3 fibroblasts in conduction modulation; no clinical implications from this in vitro feasibility study.
Key Points
- This research investigates whether transfected fibroblasts expressing Kv1.3 can alter the electrophysiological properties of cardiomyocytes.
- Utilized high-resolution multielectrode mapping to assess neonatal rat ventricular myocytes' properties
- Examined the effects of Kv1.3-expressing fibroblasts on cardiomyocytic cultures
- Applied Charybdotoxin to evaluate reversibility of electrophysiological changes
- Significant reduction in extracellular signal amplitude and emergence of multiple local conduction blocks
- Electrophysiological changes were reversed with Charybdotoxin treatment
- Control hybrid cultures with nontransfected fibroblasts showed uniform conduction
Structured PICO
Does the addition of transfected fibroblasts expressing Kv1.3 modify the electrophysiological properties of cardiomyocytic cultures?
P
Population
In vitro study using primary cultures of neonatal rat ventricular myocytes to test the electrophysiological effects of transfected fibroblasts expressing Kv1.3.
I
Intervention
Transfected fibroblasts expressing the voltage-sensitive potassium channel Kv1.3 added to the cardiomyocytic cultures
C
Comparator
Nontransfected fibroblasts added to the cardiomyocytic cultures (control hybrid cultures)
O
Outcome
Electrophysiological and structural properties (conduction properties, extracellular signal amplitude, local conduction blocks)surrogate
Transfected cellular grafts expressing ionic channels can electrically couple with cardiac myocytes to locally and reversibly modify tissue electrophysiological properties, suggesting a novel cell therapy strategy for arrhythmias.
Cite This Study
Feld et al. (2002) studied Cardiac arrhythmias (in vitro model). Transfected fibroblasts expressing the voltage-sensitive potassium channel Kv1.3 vs. Nontransfected fibroblasts was evaluated on Electrophysiological and structural properties (extracellular signal amplitude and local conduction blocks). Transfected fibroblasts expressing Kv1.3 caused a significant, reversible reduction in extracellular signal amplitude and multiple local conduction blocks in cardiomyocytic cultures.