Key result
Emv-18 transcription was highest in the spleen and bone marrow of B6.S (Fv-2ss) mice, supporting chromosomal location as an important factor controlling its expression.
Hypothesis-generating for Fv-2 control of ecotropic retrovirus in mice; leaves open translational relevance pending validation.
Expression of endogenous retroviral sequences in Fv-2 congenic mouse strains was examined by Northern blot analysis. Endogenous ecotropic virus transcripts were observed in total spleen RNA of B6.S (Fv-2ss) mice. Endogenous ecotropic transcripts were not detected in spleen RNA of C57BL/6, the Fv-2rr congenic partner of B6.S, nor in spleens of the C57BL/10 (Fv-2rr) and B10.C (Fv-2ss) congenic strains. Mendelian segregation analysis revealed that only backcross mice segregating the newly acquired Fv-2-linked endogenous ecotropic provirus had endogenous ecotropic transcripts in spleen RNA. Examination of different tissues of B6.S mice showed that Emv-18 transcription was highest in spleen and bone marrow, tissues in which Fv-2 has been shown to function. These results support the conclusion that chromosomal location is an important factor controlling Emv-18 expression in B6.S mice. We also report the presence in the spleen of a novel xenotropic virus transcript detectable only in B6.S mice.
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Robson et al. (1985) studied Fv-2 congenic mice. Fv-2 congenic mouse strains (B6.S) vs. C57BL/6, C57BL/10, and B10.C congenic strains was evaluated on Expression of endogenous retroviral sequences (Emv-18 transcription). Emv-18 transcription was highest in the spleen and bone marrow of B6.S (Fv-2ss) mice, supporting chromosomal location as an important factor controlling its expression.
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