Key result
An engineered deletion in the 3' noncoding region of human rhinovirus RNA reduced binding of a specific cellular protein and resulted in a defective viral replication phenotype in vivo.
Population
Human rhinovirus 14-infected cells and poliovirus-infected cells (in vitro models)
Comparison
In vitro UV cross-linking assay and RNA… vs Wild-type 3' NCR
Design
Preclinical
Authors
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May guide rhinovirus antiviral design targeting host interactions; leaves open clinical translation and broader picornavirus applicability.
Identification of a cellular protein that interacts with the 3' NCR of human rhinovirus and poliovirus genomic RNA suggests its role in viral RNA replication complex assembly.
Todd et al. (1995) studied Picornavirus infection (human rhinovirus and poliovirus). Engineered deletion in the 3' noncoding region (3' NCR) vs. Wild-type 3' NCR was evaluated on RNA-protein cross-linking efficiency and viral replication phenotype. An engineered deletion in the 3' noncoding region of human rhinovirus RNA reduced binding of a specific cellular protein and resulted in a defective viral replication phenotype in vivo.
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