Identification of cyclic nucleotide derivatives synthesized for radioimmunoassay development by fast atom bombardment with collision‐induced dissociation and mass‐analysed ion kinetic energy spectroscopy

The syntheses of 2′‐ O ‐succinyl, 2′‐ O ‐succinyltyrosinyl methyl ester and 2′‐ O ‐succinyliodotyrosinyl methyl ester derivatives of a cyclic nucleotide, derivatives necessary for the successful development of a specific radioimmuno‐assay, are described. Fast atom bombardment with collision‐induced dissociation and mass‐analysed ion kinetic energy spectroscopy were used to verify the positions of substitution and the retention of the 3′,5′‐cyclic phosphate moiety. Comparison of spectra produced after different iodination times permitted the optimization of the reaction conditions.