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This review analyzes the self-excising protein technology used for coordinated multi-gene expression.

August 2, 2026SynBioOpen Access

Self-Excising Proteins: Dual-Intein, Intein-2A, and Intein-Ubiquitin for Coordinated Multi-Gene Expression in Synthetic Biology

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Review highlights self-excising protein technology for multi-gene expression in eukaryotes, indicating its advantages over traditional systems.

This review analyzes the self-excising protein technology used for coordinated multi-gene expression.
Developed a panel of self-excising protein modules using engineered inteins.
Examined the application of these modules in yeast, mammalian cells, and plants.
Discussed the architecture of self-excising proteins consisting of an N-terminal excising domain and C-terminal excising domain.
Self-excising protein modules effectively release multiple proteins from a polyprotein precursor with nearly stoichiometric expression.
The approach demonstrates clean cleavage, addressing limitations of conventional 2A systems.
Coordinated coexpression using self-excising proteins has been validated in several eukaryotic systems.

Lau et al. (2026) studied this question.

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Review highlights self-excising protein technology for multi-gene expression in eukaryotes, indicating its advantages over traditional systems.