Key result
Self-processing polyproteins comprising fluorescent proteins flanking the 2A oligopeptide allowed a second protein lacking a signal sequence to slipstream through the translocon into the ER.
Population
mammalian cell lines (preclinical model)
Design
Preclinical
Authors
Loading...
Does not alter clinical practice; extends 2A-based multi-protein targeting tools in cardiovascular animal models.
The 2A self-cleaving peptide system provides a novel method for co-expressing and targeting multiple discrete proteins to different subcellular sites from a single open reading frame.
Felipe et al. (2004) studied this question. Self-processing polyproteins containing multiple signal sequences flanking 2A was evaluated on Protein translocation into the endoplasmic reticulum. Self-processing polyproteins comprising fluorescent proteins flanking the 2A oligopeptide allowed a second protein lacking a signal sequence to slipstream through the translocon into the ER.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: