January 1, 1997Electrophoresis
Apolipoprotein AI isoforms in serum determined by isoelectric focusing and immunoblotting
Key result
Quantitative evaluation of serum apolipoprotein AI isoforms revealed a qualitatively invariant pattern, with peak 3 (apo AIzero) accounting for about 74% of the total densitometric area.
Population
54 serum samples obtained from 27 men and 27 women
Design
Other
Authors
Discussion
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Implication
Validates isoelectric focusing for apo AI isoform quantification; leaves open its role in coronary heart disease risk assessment.
Study Design
Type
Observational (n=54)
Structured PICO
P
Population
54 serum samples from 27 men and 27 women analyzed for apolipoprotein AI isoforms.
E
Exposure
Isoelectric focusing (IEF) and immunoblotting
O
Outcome
Quantitative evaluation of serum apolipoprotein AI (apo AI) isoformssurrogate
Isoelectric focusing combined with immunoblotting provides a reliable method for quantifying serum apolipoprotein AI isoforms, which may have implications for coronary heart disease risk assessment.
Cite This Study
Contiero et al. (1997) reported an observational. Isoelectric focusing and immunoblotting was evaluated on Apolipoprotein AI isoform distribution. Quantitative evaluation of serum apolipoprotein AI isoforms revealed a qualitatively invariant pattern, with peak 3 (apo AIzero) accounting for about 74% of the total densitometric area.