Key result
Exposure of primary rat myotubes to human myasthenic serum produced gross morphological changes and an apparent rearrangement of the beta-cytoplasmic actin-containing cytoskeleton.
Why the study?
Does exposure to human myasthenic serum alter the distribution of beta-cytoplasmic actin and acetylcholine receptors in primary rat muscle cultures?
Population
Primary rat muscle cultures (myotubes)
Comparison
Exposure to human myasthenic serum (anti-AChR) vs Normal (unexposed) primary rat muscle cultures
Design
Preclinical
Authors
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Actin colocalizes with AChR clusters in vitro; leaves open whether cytoskeletal targeting alters neuromuscular transmission in vivo.
Does exposure to human myasthenic serum alter the distribution of beta-cytoplasmic actin and acetylcholine receptors in primary rat muscle cultures?
In primary rat muscle cells grown in vitro, acetylcholine receptors and beta-cytoplasmic actin-containing structures appear to be connected, as evidenced by their co-localization and joint rearrangement upon exposure to myasthenic serum.
B W Lubit (1984) studied this question. Human myasthenic serum was evaluated on Morphological changes and rearrangement of cytoplasmic actin-containing cytoskeleton. Exposure of primary rat myotubes to human myasthenic serum produced gross morphological changes and an apparent rearrangement of the beta-cytoplasmic actin-containing cytoskeleton.
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