What question did this study set out to answer?

To identify and characterize novel mammalian calcium-dependent protease family members distinct from previously known ubiquitous μ- and m-type isozymes.

November 1, 1989Journal of Biological ChemistryOpen Access

Molecular cloning of a novel mammalian calcium-dependent protease distinct from both m- and μ-types

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Authors

HSHiroyuki SorimachiHoward Hughes Medical Institute SIShinobu Imajoh‐OhmiThe University of Tokyo YEYasufumi EmoriKanazawa Medical University

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Implication

Molecular cloning reveals a novel calcium-dependent protease designated p94 in skeletal muscle, indicating a tissue-specific physiological role distinct from ubiquitous calpains.

Key Points

To identify and characterize novel mammalian calcium-dependent protease family members distinct from previously known ubiquitous μ- and m-type isozymes.
Screened human and rat muscle cDNA libraries using a cDNA probe derived from the chicken calcium-dependent protease large subunit.
Evaluated tissue-specific expression patterns of the isolated clone across various mammalian tissues using Northern blot analysis.
Identified a novel 821-amino-acid large subunit designated p94 (Mr 94,084) exhibiting 54% sequence homology to human μ-type and 51% to m-type large subunits.
Identified conserved cysteine protease (domain II) and calcium-binding (domain IV) domains, alongside a divergent domain I and two unique insertions of 62 and 77 residues in domains II and III.
Demonstrated exclusive transcript expression in skeletal muscle, with undetectable levels in cardiac muscle and smooth muscle tissues.

Cite This Study

Sorimachi et al. (1989) studied this question.

synapsesocial.com/papers/6a6f1ed78031ec7bb1db9a64 https://doi.org/10.1016/s0021-9258(19)47225-6

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Implication

Molecular cloning reveals a novel calcium-dependent protease designated p94 in skeletal muscle, indicating a tissue-specific physiological role distinct from ubiquitous calpains.

Molecular cloning of a novel mammalian calcium-dependent protease distinct from both m- and μ-types

Discussion

Member takes

Implication

Key Points

Cite This Study

Molecular cloning of a novel mammalian calcium-dependent protease distinct from both m- and μ-types

Discussion

Member takes

Implication

Key Points

Cite This Study

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