What question did this study set out to answer?

To isolate, clone, and functionally characterize intrahepatic hepatitis B virus nucleoprotein (HBcAg)-specific CD4+ helper T cell lines from patients with chronic active hepatitis B.

July 1, 1987The Journal of Immunology

Functional characterization of cloned intrahepatic, hepatitis B virus nucleoprotein-specific helper T cell lines.

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Authors

CFCarlo FerrariUniversity of Verona MMMario U. MondelliUniversity of Pavia APAmalia PennaScripps Research Institute

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Implication

In vitro study demonstrates functional hepatitis B nucleoprotein-specific helper T cells derived from liver tissue, indicating their direct role in local antiviral immunity and tissue injury.

Key Points

To isolate, clone, and functionally characterize intrahepatic hepatitis B virus nucleoprotein (HBcAg)-specific CD4+ helper T cell lines from patients with chronic active hepatitis B.
Cloned an established intrahepatic polyclonal T cell line from a patient with chronic active hepatitis B using limiting dilution techniques.
Assessed antigen specificity, HLA class II restriction, interleukin-2 dependence, interferon-gamma production, and helper support for autologous B cell anti-HBc synthesis.
Generated four HBcAg-specific CD3+ CD4+ CD8- DR+ T cell clones (95.3% monoclonality likelihood) exhibiting dose-dependent, HLA class II (DR)-restricted proliferation to HBcAg, without reactivity to HBsAg or pre-S(2)Ag.
All cloned helper T cell lines exhibited interleukin-2 dependence, secreted interferon-gamma in an antigen-dependent manner, and successfully induced autologous B cells to produce anti-HBc antibodies.

Cite This Study

Ferrari et al. (1987) studied this question.

synapsesocial.com/papers/6a6f2a01e71d69abee07e236 https://doi.org/10.4049/jimmunol.139.2.539

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Implication

In vitro study demonstrates functional hepatitis B nucleoprotein-specific helper T cells derived from liver tissue, indicating their direct role in local antiviral immunity and tissue injury.

Functional characterization of cloned intrahepatic, hepatitis B virus nucleoprotein-specific helper T cell lines.

Discussion

Member takes

Implication

Key Points

Cite This Study

Functional characterization of cloned intrahepatic, hepatitis B virus nucleoprotein-specific helper T cell lines.

Discussion

Member takes

Implication

Key Points

Cite This Study

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