What question did this study set out to answer?

To determine the individual contributions of Staphylococcus aureus coagulase and clumping factor to bacterial adherence and the development of infective endocarditis.

December 1, 1995Infection and ImmunityOpen Access

Role of Staphylococcus aureus coagulase and clumping factor in pathogenesis of experimental endocarditis

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Authors

PMP. MoreillonUniversité Claude Bernard Lyon 1 JEJosé M. EntenzaUniversity of Lausanne PFP FrancioliUniversity of Lusaka

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Implication

Experimental study demonstrates that clumping factor, but not coagulase, promotes heart valve colonization in a rat endocarditis model, highlighting its critical role in bacterial pathogenesis.

Key Points

To determine the individual contributions of Staphylococcus aureus coagulase and clumping factor to bacterial adherence and the development of infective endocarditis.
Tested wild-type Staphylococcus aureus Newman and isogenic mutants lacking coagulase, clumping factor, or both for in vitro attachment to rat fibrinogen and platelet-fibrin clots.
Inoculated rats bearing catheter-induced aortic vegetations with parental, mutant, or genetically complemented strains at 40% and 80% infectious doses (ID40 and ID80) to measure endocarditis induction.
Clumping factor-deficient mutants exhibited up to 100-fold reduced adherence to fibrinogen and significantly diminished binding to platelet-fibrin clots in vitro, whereas coagulase mutants showed normal adherence.
In vivo, clumping factor mutants caused approximately 50% fewer cases of endocarditis than the parent strain at ID80 (P < 0.05), a defect reversed by gene complementation, while coagulase mutants retained full infectivity.

Cite This Study

Moreillon et al. (1995) studied this question.

synapsesocial.com/papers/6a6f2dc63ce530166bc29543 https://doi.org/10.1128/iai.63.12.4738-4743.1995

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Implication

Experimental study demonstrates that clumping factor, but not coagulase, promotes heart valve colonization in a rat endocarditis model, highlighting its critical role in bacterial pathogenesis.

Role of Staphylococcus aureus coagulase and clumping factor in pathogenesis of experimental endocarditis

Discussion

Member takes

Implication

Key Points

Cite This Study

Role of Staphylococcus aureus coagulase and clumping factor in pathogenesis of experimental endocarditis

Discussion

Member takes

Implication

Key Points

Cite This Study

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