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May 1, 1985Applied Optics

Flow-cytometric light scattering measurement of red blood cell volume and hemoglobin concentration

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Authors

DTD. TyckoUniversity of California, San FranciscoMMMichael MetzUniversity of RegensburgEEEli EpsteinChildren's Hospital of Philadelphia

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Implication

Methodological study demonstrates simultaneous optical measurement of red blood cell volume and hemoglobin concentration, highlighting an accurate single-cell hematology tool.

Key Points

  • To establish and validate a dual-angle light scattering method for simultaneously quantifying individual red blood cell volume and hemoglobin concentration.
  • Modeled sphered red blood cells as homogeneous dielectric spheres with complex refractive indices linearly related to hemoglobin concentration.
  • Applied Mie scattering theory to compute reference conversion tables connecting dual-angle light scattering measurements to cell volume and hemoglobin concentration.
  • Calibrated the optical apparatus using water-immiscible oil droplets with known refractive indices and compared performance to hematological reference standards.
  • Dual-angle scatter detection successfully resolved cell volume and hemoglobin concentration simultaneously on individual sphered cells without destructive processing.
  • Experimental measurements of red blood cell populations showed direct agreement with established hematological reference measurements.

Cite This Study

Tycko et al. (1985) studied this question.

synapsesocial.com/papers/6a6f4333f44fa9f079dcad8fhttps://doi.org/10.1364/ao.24.001355
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