What question did this study set out to answer?

To evaluate the efficiency of small oligo-5'-nucleotides as initiator substrates in terminal deoxyribonucleotidyl transferase-catalyzed chain extension reactions.

November 1, 1966Biochemistry

Incorporation Efficiency of Small Oligo-5'-nucleotide Initiators in the Terminal Deoxyribonucleotide Transferase Reaction*

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Authors

FHF. Newton HayesLawrence Livermore National Laboratory VMV.E. MitchellLos Alamos National Laboratory RRRobert L. RatliffLos Alamos National Laboratory

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Implication

Biochemical assay demonstrates the incorporation efficiency of small oligo-5'-nucleotide initiators in terminal transferase reactions, suggesting structural limits for enzymatic priming.

Key Points

To evaluate the efficiency of small oligo-5'-nucleotides as initiator substrates in terminal deoxyribonucleotidyl transferase-catalyzed chain extension reactions.
Assayed terminal deoxyribonucleotidyl transferase activity using various small oligo-5'-nucleotide species as priming substrates in in vitro enzymatic reaction systems.
Small oligo-5'-nucleotide initiators support enzymatic chain elongation with efficiency governed by chain length and base composition.
Initiator characteristics directly modulate the kinetics and extent of deoxyribonucleotide polymer assembly.

Cite This Study

Hayes et al. (1966) studied this question.

synapsesocial.com/papers/6a6f446f78a11c550e08f396 https://doi.org/10.1021/bi00875a035

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Implication

Biochemical assay demonstrates the incorporation efficiency of small oligo-5'-nucleotide initiators in terminal transferase reactions, suggesting structural limits for enzymatic priming.

Incorporation Efficiency of Small Oligo-5'-nucleotide Initiators in the Terminal Deoxyribonucleotide Transferase Reaction*

Discussion

Member takes

Implication

Key Points

Cite This Study

Incorporation Efficiency of Small Oligo-5'-nucleotide Initiators in the Terminal Deoxyribonucleotide Transferase Reaction*

Discussion

Member takes

Implication

Key Points

Cite This Study

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