What question did this study set out to answer?

To elucidate the molecular mechanisms by which Polycomb group proteins repress transcription and how their target genes become activated.

April 12, 2010Nucleic Acids ResearchOpen Access

Characterization of an antagonistic switch between histone H3 lysine 27 methylation and acetylation in the transcriptional regulation of Polycomb group target genes

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Authors

DPDiego PasiniUniversity of Milan MMMartina MalatestaBristol-Myers Squibb (United States)HJHye Ryung JungUniversity of Copenhagen

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Implication

Mechanistic study reveals that loss of PRC2 switches H3K27 methylation to acetylation in stem cells, indicating Polycomb proteins repress genes by blocking acetyltransferase binding.

Key Points

To elucidate the molecular mechanisms by which Polycomb group proteins repress transcription and how their target genes become activated.
Determined global alterations in histone modifications in embryonic stem cells lacking the essential Polycomb repressive complex 2 subunit Suz12.
Tracked histone modification dynamics during embryonic stem cell differentiation and in MLL-AF9-transduced hematopoietic stem cells.
Assessed the functional contribution of the acetyltransferases p300 and CBP to H3K27 acetylation.
Loss of PRC2 activity via Suz12 deficiency triggered a global increase in H3K27 acetylation.
The switch from H3K27 methylation to acetylation correlated directly with transcriptional activation of Polycomb target genes during differentiation and leukemogenesis.
The acetyltransferases p300 and CBP catalyzed H3K27 acetylation, demonstrating that Polycomb proteins maintain repression in part by preventing acetyltransferases from accessing target genes.

Cite This Study

Pasini et al. (2010) studied this question.

synapsesocial.com/papers/6a6f45a122b3175134db425c https://doi.org/10.1093/nar/gkq244

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Implication

Mechanistic study reveals that loss of PRC2 switches H3K27 methylation to acetylation in stem cells, indicating Polycomb proteins repress genes by blocking acetyltransferase binding.

Characterization of an antagonistic switch between histone H3 lysine 27 methylation and acetylation in the transcriptional regulation of Polycomb group target genes

Discussion

Member takes

Implication

Key Points

Cite This Study

Characterization of an antagonistic switch between histone H3 lysine 27 methylation and acetylation in the transcriptional regulation of Polycomb group target genes

Discussion

Member takes

Implication

Key Points

Cite This Study

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