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September 1, 1989Journal of Cell Science

Repetitive titin epitopes with a 42 nm spacing coincide in relative position with known a band striations also identified by major myosin-associated proteins an immunoelectron-microscoplcal study on myofibrils

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Population

Myofibrils

Design

Preclinical

Authors

DFDieter O. FürstHeart Failure & TransplantRNRüdiger NaveMax Planck SocietyMOMary OsbornGoethe University Frankfurt

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Implication

Extends titin epitope mapping in animal sarcomeres; leaves open functional roles in human cardiac disease.

Key Points

  • This research aims to explore the interaction between titin and myosin proteins within the A band of myofibrils.
  • Utilized four monoclonal antibodies specific for titin in immunoelectron microscopy.
  • Identified quasi-repeats in the titin molecule with a repeat spacing of 42-43 nm.
  • Examined negatively stained cryosections of the A band to correlate titin localization with known striations.
  • Antibodies T30 and T32 recognized five or two of the seven known striations per half sacromere (location of C-protein and 86K protein).
  • The T33 epitope is located 55 nm before the center of the M band, consistent with the M7 striation.
  • Titin's structure indicates a potential interaction pattern with thick filaments in the sarcomere.

Structured PICO

P
Population
Myofibrils
I
Intervention
Four new monoclonal antibodies specific for titin (T30, T31, T32, T33) in immunoelectron microscopy
O
Outcome
Localization of titin epitopes in the A band and M band of the sarcomeresurrogate

The study maps specific titin epitopes to known ultrastructural striations in the A and M bands, suggesting a regular interaction pattern between titin and thick filaments.

Cite This Study

Fürst et al. (1989) studied this question.

synapsesocial.com/papers/6a6f47ce31a3df824327afbahttps://doi.org/10.1242/jcs.94.1.119
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Extensible and Less-Extensible Domains of Connectin Filaments in Stretched Vertebrate Skeletal Muscle Sarcomeres as Detected by Immunofluorescence and Immunoelectron Microscopy Using Monoclonal Antibodies11988 · 149 citations
  2. 2A Comparative Study of High Molecular Weight Proteins in Various Types of Muscle Across the Animal Kingdom1986 · 92 citations
  3. 3The location of C-protein in rabbit skeletal muscle1976 · 256 citations
  4. 4Monoclonal antibodies distinguish titins from heart and skeletal muscle.1986 · 50 citations
  5. 5Architecture of the sarcomere matrix of skeletal muscle: immunoelectron microscopic evidence that suggests a set of parallel inextensible nebulin filaments anchored at the Z line.1988 · 264 citations