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November 1, 1989The Journal of Cell BiologyOpen Access

Visualization of the polarity of isolated titin molecules: a single globular head on a long thin rod as the M band anchoring domain?

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Key result

Isolated titin molecules consist of a single globular head on a long, thin rod of uniform diameter (900 nm), which likely corresponds to the M band anchoring domain.

Population

Isolated titin molecules (TII) purified from chicken breast muscle myofibrils

Design

Preclinical

Authors

RNRüdiger NaveMax Planck SocietyDFDieter O. FürstHeart Failure & TransplantKWK. WeberPsychiatry Baselland

Discussion

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Overview

Novel titin visualization extends sarcomere structural maps; leaves open functional roles in cardiac disease.

Structured PICO

P
Population
Isolated titin molecules (TII) purified from chicken breast muscle myofibrils
E
Exposure
Purification by high resolution gel permeation chromatography into TIIA and TIIB fractions, followed by novel specimen orientation methods (centrifugation) and electron microscopy
O
Outcome
Visualization of the structure and polarity of titin molecules

Isolated titin molecules possess a polar structure with a single globular head that likely anchors to the M band of the sarcomere.

Cite This Study

Nave et al. (1989) studied this question. Titin purification and electron microscopy was evaluated on Molecular structure of isolated titin molecules. Isolated titin molecules consist of a single globular head on a long, thin rod of uniform diameter (900 nm), which likely corresponds to the M band anchoring domain.

synapsesocial.com/papers/6a6c8ea635aa2c282cdf9700https://doi.org/10.1083/jcb.109.5.2177
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Also Consider

Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Repetitive titin epitopes with a 42 nm spacing coincide in relative position with known a band striations also identified by major myosin-associated proteins an immunoelectron-microscoplcal study on myofibrils1989 · 110 citations
  2. 2Titin: major myofibrillar components of striated muscle.1979 · 548 citations
  3. 3Connectin filaments link thick filaments and Z lines in frog skeletal muscle as revealed by immunoelectron microscopy.1985 · 141 citations
  4. 4Preparation of Native Connectin from Chicken Breast Muscle11983 · 44 citations