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December 23, 2010Journal of VirologyOpen Access

A Single Amino Acid Substitution in the Capsid of Foot-and-Mouth Disease Virus Can Increase Acid Resistance

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Key result

A single amino acid substitution (N17D) at the N terminus of the VP1 capsid protein increased the resistance of foot-and-mouth disease virus to acid-induced inactivation and capsid dissociation.

Population

Foot-and-mouth disease virus (FMDV) variants (six variants and parental C-S8c1 virus)

Comparison

Amino acid replacement N17D at the N terminus of… vs Parental C-S8c1 virus

Design

Preclinical

Authors

MMMiguel A. Martín-AcebesConsejo Superior de Investigaciones CientíficasÁVÁngela Vázquez-CalvoCentro de Biología Molecular Severo OchoaVRVerónica RincónUniversidad Nacional de Colombia

Discussion

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Implication

Supports capsid engineering for acid-stable FMDV; leaves open translation to field strains or livestock trials.

Structured PICO

P
Population
In vitro virology study investigating the molecular determinants of foot-and-mouth disease virus resistance to acid-induced disassembly using isolated viral variants.
I
Intervention
Amino acid replacement N17D at the N terminus of VP1 capsid protein
C
Comparator
Parental C-S8c1 virus
O
Outcome
Resistance to acid-induced disassembly/inactivationsurrogate

A single amino acid substitution (N17D) in the FMDV capsid increases viral resistance to acid-induced disassembly, illustrating the adaptive flexibility of viral quasispecies.

Limitations

  • In vitro study design
  • The biological effect of the additional VP3 D9V mutation requires further experiments to fully determine its role

Cite This Study

Martín-Acebes et al. (2010) studied Foot-and-mouth disease virus (FMDV). Amino acid replacement N17D at the N terminus of VP1 capsid protein vs. Parental C-S8c1 virus was evaluated on Resistance to acid-induced inactivation and capsid dissociation. A single amino acid substitution (N17D) at the N terminus of the VP1 capsid protein increased the resistance of foot-and-mouth disease virus to acid-induced inactivation and capsid dissociation.

synapsesocial.com/papers/6a6f57e3fe4101aa97dfc187https://doi.org/10.1128/jvi.02245-10
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1A Single Amino Acid Substitution in the Capsid of Foot-and-Mouth Disease Virus Can Increase Acid Lability and Confer Resistance to Acid-Dependent Uncoating Inhibition2010 · 47 citations
  2. 2Genome Delivery and Ion Channel Properties Are Altered in VP4 Mutants of Poliovirus2003 · 127 citations
  3. 3Lessons from the foot and mouth disease outbreak in the Netherlands in 20012002 · 98 citations
  4. 4Complete Alanine Scanning of Intersubunit Interfaces in a Foot-and-Mouth Disease Virus Capsid Reveals Critical Contributions of Many Side Chains to Particle Stability and Viral Function2003 · 55 citations
  5. 5A Dominant-Negative Mutant of rab5 Inhibits Infection of Cells by Foot-and-Mouth Disease Virus: Implications for Virus Entry2009 · 68 citations