Key result
Alanine scanning of 42 interfacial side chains in the foot-and-mouth disease virus capsid showed that most mutations drastically reduced virus yields and altered thermal inactivation rates.
Population
Foot-and-mouth disease virus capsid model
Comparison
Individual truncation to alanine of 42 amino… vs Parent virus
Design
Preclinical
Authors
Loading...
Pinpoints tolerant capsid sites for FMDV vaccine engineering; leaves open in vivo validation and translational utility.
Most residues at the interfaces between foot-and-mouth disease virus capsid subunits are critically important for viral function and stability, though a few are tolerant to mutation and could be targets for engineering thermostable vaccines.
Mateo et al. (2003) studied Foot-and-mouth disease virus. Alanine scanning mutagenesis of 42 amino acid side chains vs. Parent virus was evaluated on Virus yields, thermal inactivation rates, and viability. Alanine scanning of 42 interfacial side chains in the foot-and-mouth disease virus capsid showed that most mutations drastically reduced virus yields and altered thermal inactivation rates.
Synapse has enriched 3 closely related papers on similar clinical questions. Consider them for comparative context: