Key result
Picornaviruses were detected in 33.86% of faecal samples from diarrhoeic calves, including bovine kobuvirus (22.83%), bovine hungarovirus (11.02%), and bovine enterovirus 1 (5.51%).
Why the study?
The involvement of picornaviruses in calf diarrhoea required evaluation.
Cross-Sectional (n=127)
This study provides the first molecular evidence of bovine hungarovirus, bovine kobuvirus, and bovine enterovirus 1 in diarrhoeic calves in Turkey, suggesting they may be aetiological agents of calf diarrhoea.
Hypothesis-generating for picornavirus role in calf diarrhoea; prospective studies needed before diagnostic or management changes.
The involvement of picornaviruses in calf diarrhoea was evaluated by the analysis of 127 faecal samples collected from diarrhoeic calves during 2014–2016. Virus detections were carried out by PCR using generic or specific primer pairs. One-third of the faecal samples (33.86%) were found to be positive for one or more of the studied viruses. Bovine kobuvirus was detected in 22.83%, bovine hungarovirus in 11.02%, while bovine enterovirus 1 in 5.51% of the samples. The sequences of the PCR products indicated the existence of novel variants in all the three virus species. When comparing the partial sequences, the nucleotide sequence identities between our newly detected viruses and those previously deposited to the GenBank ranged between 76 and 99%. Phylogenetic analyses revealed a novel lineage within the species Hunnivirus A. Our findings suggest that these viruses should be regarded as possible aetiological agents of calf diarrhoea. Based on the newly determined sequences, we designed and tested a new generic PCR primer set for the more reliable detection of bovine hungaroviruses. This is the first report on the molecular detection of the presence of bovine hungarovirus, bovine kobuvirus and bovine enterovirus 1 in the faecal samples of diarrhoeic calves in Turkey.
No takes yet. Share an insight, caveat, or question.
Işıdan et al. (2019) conducted a cross-sectional in Calf diarrhoea (n=127). Picornavirus infection was evaluated on Detection of picornaviruses. Picornaviruses were detected in 33.86% of faecal samples from diarrhoeic calves, including bovine kobuvirus (22.83%), bovine hungarovirus (11.02%), and bovine enterovirus 1 (5.51%).
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: