What question did this study set out to answer?

To isolate and characterize a cyclic AMP-independent protein kinase from rabbit skeletal muscle that catalyzes the total conversion of glycogen synthase from the I-form to the D-form.

February 1, 1977Journal of Biological ChemistryOpen Access

Total conversion of glycogen synthase from the I- to the D-form by a cyclic AMP-independent protein kinase from rabbit skeletal muscle.

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Authors

EIEmilio ItarteUniversitat Autònoma de Barcelona JRJ.C. RobinsonUniversity of California, Berkeley KHKuo‐Ping HuangMinistry of Health and Welfare

Discussion

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Implication

Biochemical analysis demonstrates total conversion of glycogen synthase from I- to D-form by a novel protein kinase in rabbit muscle, indicating an alternative pathway for enzyme regulation.

Key Points

To isolate and characterize a cyclic AMP-independent protein kinase from rabbit skeletal muscle that catalyzes the total conversion of glycogen synthase from the I-form to the D-form.
Purified and resolved glycogen synthase kinase from cyclic AMP-dependent protein kinase using phosphocellulose column chromatography.
Assessed enzyme substrate specificity, sensitivity to cyclic AMP and its kinase inhibitor, and phosphorylation stoichiometry against I-form glycogen synthase.
Glycogen synthase kinase catalyzed the incorporation of 4 mol of phosphate per 85,000-dalton subunit of I-form glycogen synthase.
Phosphorylation at two specific sites predominantly governed glucose-6-phosphate dependence, producing a multiply phosphorylated enzyme equivalent in phosphate content to the muscle D-form.

Cite This Study

Itarte et al. (1977) studied this question.

synapsesocial.com/papers/6a6f6a8875498292b708cf27 https://doi.org/10.1016/s0021-9258(17)40645-4

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Implication

Biochemical analysis demonstrates total conversion of glycogen synthase from I- to D-form by a novel protein kinase in rabbit muscle, indicating an alternative pathway for enzyme regulation.

Total conversion of glycogen synthase from the I- to the D-form by a cyclic AMP-independent protein kinase from rabbit skeletal muscle.

Discussion

Member takes

Implication

Key Points

Cite This Study

Total conversion of glycogen synthase from the I- to the D-form by a cyclic AMP-independent protein kinase from rabbit skeletal muscle.

Discussion

Member takes

Implication

Key Points

Cite This Study

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