What question did this study set out to answer?

To determine the amino acid sequence and structural localization of seven serine phosphorylation sites targeted by five protein kinases on rabbit skeletal muscle glycogen synthase.

May 1, 1982European Journal of Biochemistry

Multisite Phosphorylation of Glycogen Synthase from Rabbit Skeletal Muscle

View Full Paper

Ask AI

Bookmark

Authors

CPColin PictonSea Turtle Conservancy AAAlastair AitkenUniversity of London TBTerry BILHAMBiogen (United States)

Discussion

Member takes

Implication

Biochemical analysis reveals the structural mapping of seven phosphorylation sites on rabbit muscle glycogen synthase, indicating mechanisms for multisite regulation in vivo.

Key Points

To determine the amino acid sequence and structural localization of seven serine phosphorylation sites targeted by five protein kinases on rabbit skeletal muscle glycogen synthase.
Sequenced the N-terminal 123 amino acid residues of cyanogen bromide peptide CB2 from rabbit skeletal muscle glycogen synthase.
Mapped phosphorylation targets for cyclic-AMP-dependent protein kinase, phosphorylase kinase, and glycogen synthase kinases 3, 4, and 5.
Identified selective proteolytic cleavage sites generated by trypsin, chymotrypsin, and thermolysin.
Site 2 is localized near the N-terminus in peptide CB1, whereas sites 3a, 3b, 3c, 5, 1a, and 1b are clustered in the C-terminal peptide CB2 at residues 30, 34, 38, 46, 87, and 100, respectively.
The phosphorylation domain within CB2 is highly hydrophilic and protease-sensitive, containing a proline-rich segment around sites 3a–3c and an acidic segment of 15 aspartic and glutamic acid residues following site 5.
Trypsin cleaves C-terminal to site 3c while chymotrypsin cleaves N-terminal to site 3a, establishing a method to monitor the in vivo phosphorylation state of all seven serine residues.

Cite This Study

Picton et al. (1982) studied this question.

synapsesocial.com/papers/6a7270543ce530166bc352cb https://doi.org/10.1111/j.1432-1033.1982.tb05903.x

View Full Paper

Ask AI

Bookmark

Implication

Biochemical analysis reveals the structural mapping of seven phosphorylation sites on rabbit muscle glycogen synthase, indicating mechanisms for multisite regulation in vivo.

Multisite Phosphorylation of Glycogen Synthase from Rabbit Skeletal Muscle

Discussion

Member takes

Implication

Key Points

Cite This Study

Multisite Phosphorylation of Glycogen Synthase from Rabbit Skeletal Muscle

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider