What question did this study set out to answer?

This research aims to elucidate the structural characteristics and ligand binding sites of glycogen phosphorylase a.

Structure of glycogen phosphorylase a at 3.0 A resolution and its ligand binding sites at 6 A.

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X-ray diffraction reveals glycogen phosphorylase a's structure and binding sites, highlighting metabolic control mechanisms.

This research aims to elucidate the structural characteristics and ligand binding sites of glycogen phosphorylase a.
Built a polypeptide model from a 3 A resolution electron density map derived from x-ray diffraction analysis.
Analyzed native tetragonal crystals and derivatives for structural insights.
Identified active and binding sites through spatial examination of the polypeptide chain.
The model reveals two active sites per dimer shared between subunits.
Identified binding sites for glucose-1-P, arsenate, UDP-glucose, and AMP within a pocket region.
Maltoheptaose binding supports the hypothesis of a polysaccharide storage site in muscle cells.

Fletterick et al. (1976) studied this question.

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