Renal proximal tubule angiotensin AT1A receptors regulate blood pressure

Mice models at 4-6 months of age: KAP2-AT1AR-N111G transgenic mice (overexpressing constitutively active AT1AR) and KAP2-AT1AR-KO mice (proximal tubule-specific depletion of AT1AR) on a C57BL/6J background.

Androgen (testosterone) administration via 10 mg subcutaneous pellet for 10 days to induce proximal tubule-specific AT1AR overexpression or deletion.

Nontransgenic littermate controls and/or mice without testosterone administration.

Systemic blood pressure (systolic, diastolic, mean arterial pressure) measured by tail cuff plethysmography and radiotelemetry.surrogate