What question did this study set out to answer?

The aim is to identify which regions of the alpha 1-adrenergic receptor are critical for activating phosphatidylinositol metabolism.

Regions of the alpha 1-adrenergic receptor involved in coupling to phosphatidylinositol hydrolysis and enhanced sensitivity of biological function.

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The aim is to identify which regions of the alpha 1-adrenergic receptor are critical for activating phosphatidylinositol metabolism.
Constructed mutant alpha 1-adrenergic receptors using recombinant DNA techniques.
Tested a chimeric receptor with the third cytoplasmic loop of the alpha 1AR for functionality in phosphatidylinositol metabolism.
Analyzed the effects of specific amino acid substitutions on G protein coupling and receptor sensitivity.
A chimeric receptor activated phosphatidylinositol metabolism as effectively as the native alpha 1AR.
Conservative substitutions in the C terminus of the third cytoplasmic loop improved agonist potency for phosphatidylinositol metabolism by up to 100-fold.
Mutations in the cytoplasmic tail reduced receptor coupling to phospholipase C.

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Randomized trial demonstrates enhanced sensitivity of receptor functions in mutant alpha 1-adrenergic receptors, indicating biological implications.

Cotecchia et al. (1990) studied this question.

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Randomized trial demonstrates enhanced sensitivity of receptor functions in mutant alpha 1-adrenergic receptors, indicating biological implications.