The Effects of Deletions, Point Mutations, Reversions and Suppressor Mutations on the Two Components of the Tryptophan Synthetase of Escherichia Coli

Charles Yanofsky; Irving P. Crawford

doi:10.1073/pnas.45.7.1016

July 1, 1959Proceedings of the National Academy of Sciences

The Effects of Deletions, Point Mutations, Reversions and Suppressor Mutations on the Two Components of the Tryptophan Synthetase of Escherichia Coli

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Structured PICO

P

Population

Escherichia coli strain K-12 and strain B mutants

I

Intervention

Deletions, point mutations, reversions, and suppressor mutations

C

Comparator

Wild type E. coli K-12

O

Outcome

Tryptophan synthetase component A and B enzymatic activity and immunological cross-reacting material (CRM)surrogate

Specific genic regions control the formation of the A and B proteins of tryptophan synthetase in E. coli, and point mutations can lead to either loss of protein formation or altered proteins with partial activity.

Limitations

Agreement between duplicate transduction experiments was very poor, with values sometimes varying by as much as 40 per cent
Unexpectedly small number of tryp+ colonies in reciprocal transduction experiments with mutants A-3 and A-4, for which the explanation is not known

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Implication

No immediate clinical implications; hypothesis-generating for mutational effects on protein formation in model systems.

Abstract

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Discussion

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Cite This Study

Yanofsky et al. (1959) studied this question.

synapsesocial.com/papers/6a6f7f4078a11c550e093687 https://doi.org/10.1073/pnas.45.7.1016

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The Effects of Deletions, Point Mutations, Reversions and Suppressor Mutations on the Two Components of the Tryptophan Synthetase of Escherichia Coli

Structured PICO

Limitations

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

The Effects of Deletions, Point Mutations, Reversions and Suppressor Mutations on the Two Components of the Tryptophan Synthetase of Escherichia Coli

Structured PICO

Limitations

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

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