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January 1, 1989Nucleic Acids ResearchOpen Access

Efficient oligonucleotide-directed construction of mutations in expression vectors by the gapped duplex DNA method using alternating selectable markers

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Authors

PSPatrick StanssensAblynx (Belgium)COChris OpsomerVIB-UGent Center for Plant Systems BiologyYMYvonne M. McKeownVIB-UGent Center for Plant Systems Biology

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Stanssens et al. (1989) studied this question.

synapsesocial.com/papers/6a6f8b6da528af2d65c30b0chttps://doi.org/10.1093/nar/17.12.4441
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Also Consider

Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Escherichia coli mutator mutants deficient in methylation-instructed DNA mismatch correction.1980 · 401 citations
  2. 2A new method for sequencing DNA.1977 · 8,033 citations
  3. 3Oligonucleotide-directed construction of mutations: a gapped duplex DNA procedure without enzymatic reactionsin vitro1988 · 20 citations
  4. 4Rapid and efficient site-specific mutagenesis without phenotypic selection.1985 · 7,328 citations