What question did this study set out to answer?

To identify the discrete DNA regulatory elements governing muscle- and fiber type-specific transcription of murine and human myoglobin genes.

May 1, 2001Journal of Biological ChemistryOpen Access

Regulatory Elements Governing Transcription in Specialized Myofiber Subtypes

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Authors

ZYZhen YanBoston University ASAntonio L. SerranoFleet Science Center SSStefano SchiaffinoGeneral Cardiology

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Implication

Molecular genetic analysis reveals regulatory DNA elements controlling myoglobin expression across skeletal myofiber subtypes, highlighting an intragenic E-box that acts as a negative regulator.

Key Points

To identify the discrete DNA regulatory elements governing muscle- and fiber type-specific transcription of murine and human myoglobin genes.
Utilized germ-line transgenesis and somatic cell gene transfer to analyze murine and human myoglobin gene regulatory sequences coupled to reporter genes.
Conducted mutational analyses of upstream MEF2-binding regions and intragenic sequence motifs.
Identified discrete regulatory regions in human and murine myoglobin genes sufficient to drive reporter gene expression specifically in oxidative (Types I and IIa) over glycolytic (Type IIb) myofibers.
Confirmed that A/T-rich MEF2-binding motifs are essential for activating myoglobin gene transcription in skeletal muscle.
Demonstrated that an intragenic E-box motif functions as a negative regulatory element that represses myoglobin expression to ensure myofiber subtype specificity.

Cite This Study

Yan et al. (2001) studied this question.

synapsesocial.com/papers/6a6f92c2439bab0cabc2d7e0 https://doi.org/10.1074/jbc.m101251200

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Implication

Molecular genetic analysis reveals regulatory DNA elements controlling myoglobin expression across skeletal myofiber subtypes, highlighting an intragenic E-box that acts as a negative regulator.

Regulatory Elements Governing Transcription in Specialized Myofiber Subtypes

Discussion

Member takes

Implication

Key Points

Cite This Study

Regulatory Elements Governing Transcription in Specialized Myofiber Subtypes

Discussion

Member takes

Implication

Key Points

Cite This Study

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