June 1, 1989Journal of Biological ChemistryOpen Access

Muscle-specific Regulation of a Transfected Rabbit Myosin Heavy Chain βGene Promoter

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Population

Cultured myoblasts from 12-day-old chick embryonic breast muscle

Comparison

Transfection with a chimeric gene containing 781… vs Undifferentiated myoblasts or myoblasts blocked…

Design

Preclinical

Authors

LCLeanne L. CribbsUniversity of Iowa NSN. ShimizuKyushu Kyoritsu University CYC E YockeyHarvard University Press

Discussion

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Implication

Does not alter clinical practice; leaves open relevance of these elements to human cardiac myosin regulation.

Structured PICO

Population

Cultured myoblasts from 12-day-old chick embryonic breast muscle

Intervention

Transfection with a chimeric gene containing 781 base pairs of the rabbit myosin heavy chain beta promoter region fused to the chloramphenicol acetyltransferase (CAT) gene, progressive 5' deletions, and linker-scanner mutagenesis

Comparator

Undifferentiated myoblasts or myoblasts blocked with 5-bromodeoxyuridine

Outcome

Transcriptional activity (measured by CAT expression) and identification of regulatory elementssurrogate

The rabbit myosin heavy chain beta gene promoter is regulated in a tissue- and stage-specific manner, requiring at least two distinct elements within the -294 base pair region for muscle-specific expression.

Cite This Study

Cribbs et al. (1989) studied this question.

synapsesocial.com/papers/6a6f92c8439bab0cabc2d7f2 https://doi.org/10.1016/s0021-9258(18)81675-1

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Muscle-specific Regulation of a Transfected Rabbit Myosin Heavy Chain βGene Promoter

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Muscle-specific Regulation of a Transfected Rabbit Myosin Heavy Chain βGene Promoter

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider